Chemical Agent Turns Tissue Transparent

Screen shot 2011-09-07 at 10.14.35.png.jpegOlivia Solon writes on Wired:

Japanese researchers have developed a chemical agent that turns biological tissue transparent, allowing for vivid imaging of neurons and blood vessels deep inside mouse brains.

The aqueous reagent — referred to as Scale — offers a way of analyzing complex organs and networks in tissue samples, without having to dissect them into smaller pieces. Developed by Atsushi Miyawaki and his team at the RIKEN Brain Science Institute, Scale performs better than other clearing reagents because it doesn’t affect the shape or proportions of the sample. It also manages to avoid decreasing the strength of signals emitted by genetically-encoded fluorescent proteins in the tissue, which are frequently used by researchers as markers to flag up specific cells.

This means that neuroscientists can visualise fluorescently-labelled brain samples at a depth of several millimetres (as opposed to just one millimetre) and see neural networks at sub-cellular resolution. The team has used the agent to examine the neurons in a mouse brain and have been able to see the inner workings of the cerebral cortex, hippocampus and white matter. They have then been able to create 3D reconstructions of the neural networks, as you can see here.

[Continues at Wired]

10 Comments on "Chemical Agent Turns Tissue Transparent"

  1. unfortunately we never got this kind of financial transparency

  2. unfortunately we never got this kind of financial transparency

  3. Wanooski | Sep 9, 2011 at 6:09 pm |

    Or political.

  4. And bonus: we can turn embryo’s into gummy bears

  5. And bonus: we can turn embryo’s into gummy bears

  6. Liquidstar | Sep 9, 2011 at 8:17 pm |

    I bet this is going to be on Fringe this season.

  7. Liquidstar | Sep 9, 2011 at 4:17 pm |

    I bet this is going to be on Fringe this season.

  8. Rex Vestri | Sep 9, 2011 at 10:17 pm |

    It’s an invisible NINJA embryo!

  9. Rex Vestri | Sep 9, 2011 at 6:17 pm |

    It’s an invisible NINJA embryo!

Comments are closed.